Impact of the Expression System on Recombinant Protein Production in Escherichia coli BL21

Advances and challenges in recombinant protein expression in Escherichia coli

Cloning and Expression of Influenza H1N1 NS1 Protein in Escherichia Coli BL21

Background:Influenza virus is globally pathogenic and it is usually associated with zoonotic respiratory diseases. This virus has caused a number of pandemics with a high mortality rate. The non-structural (NS1) protein of influenza A viruses is a non-essential virulence factor that has multiple accessory functions during viral infection. This protein is highly conservative. It has been shown t...

Production of specific IgY antibody to the recombinant FanC protein produced in Escherichia coli

Objective(s): Enterotoxigenic Escherichia coli (ETEC) strains are one of the primary causes of diarrhea in newborn calves and in humans, pigs, and sheep. IgY technology has been identified as a promising alternative to generating a mass amount of specific antibody for use in immunotherapy and immunodiagnostics. The purpose of this study was to produce specific antibody by egg yolk antibody (IgY...

down regulation of acka-pta pathway in escherichia coli bl21 (de3): a step toward optimized recombinant protein expression system

materials and methods: in order to achieve this, recombinant plasmid harboring antisense sequences targeting both of pta and acka was assembled, after transfecting to the cells, its effects on the cell growth and acetate accumulation in the minimal media was assessed and compared with the control, the plasmid without antisense cassette, in presence and absence of iptg in escherichia coli bl21 (...

the impact of e-readiness on ec success in public sector in iran the impact of e-readiness on ec success in public sector in iran

acknowledge the importance of e-commerce to their countries and to survival of their businesses and in creating and encouraging an atmosphere for the wide adoption and success of e-commerce in the long term. the investment for implementing e-commerce in the public sector is one of the areas which is focused in government‘s action plan for cross-disciplinary it development and e-readiness in go...